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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Rankl genetic deficiency and functional blockade undermine skeletal stem and progenitor cell differentiation
doi: 10.1186/s13287-024-03803-3
Figure Lengend Snippet: Impact of Denosumab treatment on the mineralization capacity of human BMSCs. a Representative image of ARS-stained cultures of human BMSCs from healthy donors after 2 weeks of culture either in basal medium (CTR) or in OIM alone or in OIM + isotype control (OIM + ISO) or in OIM + Denosumab (OIM + Dmab); in all the conditions, the culture medium was changed twice a week. At the end, the mineral deposition was quantified and indicated by Abs reading at 405 nm. Scale bar: 500 μm. Higher magnifications are provided in Figure . b Gene expression analysis of representative osteogenic genes in human BMSCs in the different treatment conditions indicated; normalization on 18 S. Results are expressed as Arbitrary Units (A.U.). All the data are represented as mean ± SEM. * p < 0.05; a: Friedman test; b: Kruskal-Wallis test. CTR: control. OIM: Osteogenic Induction Medium. ISO: isotype control. Dmab: Denosumab
Article Snippet:
Techniques: Staining, Control, Expressing